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Objective To understand the main genotypes ,age distribution and infection situation of women cervical human papil‐lomavirus (HPV) infection ,and to investigate the clinical value of the HPV genotype detection combined with thinprep cytology test(TCT ) in screening the cervical lesions .Methods The detection results in 3 272 women voluntarily receiving the cervical cancer screening ,HPV genotype detection and TCT examination in the gynecology clinic of our hospital from January 2012 to December 2013 were performed the statistical analysis .Results Among 3 272 cases ,841 cases were HPV positive with the positive rate of 25 .70% and mainly concentrated in 25 - < 50 years old ,in which 714 strains were high‐risk subtype (including mixed infection) , accounting for 84 .90% ,the HPV type 16 ,52 ,58 ,18 were in the top four ,the multiple infection was in 185 cases (at least one kind of high risk type was detected out in each case of multiple infection) ;253 cases were TCT positive with the positive rate of 7 .73% , in which 167 cases were abnormal diagnosed by the pathological biopsy ,the coincidence rate reached 66 .01% ,among 167 cases of positive pathological biopsy ,166 cases were HPV infection ,7 cases of cervical cancer all were infected by high‐risk type HPV .Con‐clusion The HPV subtype detection combined with TCT can significantly increase the positive rate of cervical cancer screening , which conduces to achieve early prevention ,early discovery ,early treatment for reducing the incidence of cervical cancer .
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Objective To observe the influence of rational emotional therapy on breast cancer patients preoperation. Methods 50 cases of breast cancer patients were randomly divided into the experimental group and the control group with 25 cases in each group. The control group was provided routine therapy and nursing, health education and general psychological nursing, based upon these routine measures, the experimental group adopted systematic and standardized rational emotional therapy. Zung self rating depression scale(SDS), Zung self rating anxiety scale(SAS), Pittsburgh Sleep Quality Index scale(PSQI)were used to evaluate the effect between two groups. Results No significant differ-ence existed in anxiety, depression and sleep quality between the two groups at admission, but significant difference was seen one day preoperation, also there was statistical significance between at admission and one day preoperation in the experimental group. Conclusions Rational emotional therapy of breast can-cer patients who underwent surgical operation played a significant role on their preoperative short-term e-motions(including anxiety,depression, sleep quality), and worthy of clinical application.
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Objective To investigate the levels of platelet-derived growth factor-BB (PDGF-BB) and its role in the pathogenesis of the patients with hepatic diseases.Methods The levels of serum PDGF-BB in 30 chronic patients (hepatitis B,26; hepatitis C,4),24 patients with post-hepatitis cirrhosis,30 patients with primary hepatocellular carcinoma,and 20 normal controls were measured by ELISA.The levels of PDGF-B mRNA in peripheral blood mononuclear cells were measured by reverse transcription polymerase chain reaction (RT-PCR).Results The serum levels of PDGF-BB and the levels of PDGF-B mRNA in peripheral blood mononuclear cells were significantly higher in the patients with hepatic diseases (F=1774.40,1037.42,P
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Objective:To explore the genotype distribution of lipoprotein lipase polymorphism at Pvu Ⅱ locus and its possible association with serum lipid levels, body mass index (BMI) and dietary intake. Method:Pvu II polymorphism was determined by PCR-PFLP method in 156 adults with hyperlipidemia and 154 adults with normal serum lipid levels as control in Shanghai. Their physical examinations, dietary investigation and the levels of serum lipid profile, including TG, TC, HDL and LDL were analyzed. Results: ⑴ There was no significant difference in frequencies of genotypes or allele between the hyperlipidemia and the control, and between male and female. ⑵ BMI were significantly different between P-P-and P+P+, P+P-genotype groups. The BMI of subjects with genotype P+P+ had the highest levels. The relationship was significant after adjusting several confounding factors such as age, gender and blood lipid (P